Recombinant FGF2-G3 (FGF2-STAB®) protein is a thermostable engineered form of FGF-2 (bFGF). Qk053 is the 154 aa mature domain of FGF-2 (Qk027) with nine amino acid substitutions to enhance stability without impacting bioactivity developed by Dvorak et al. 2018. This increases the functional half-life of the protein from 10 h (wild-type) to >7 days (FGF2-G3).
Recombinant FGF2-G3 is used in B8 media (Kuo et al. 2019) for weekend free, high homogeneity induced pluripotent stem cell culture. FGF2-G3 also has applications in chemically defined stem cell and organoid culture media, and cultured meat media development.
High purity 17 kDa bioactive FGF2-G3 protein, animal origin-free (AOF), carrier protein-free and tag free.
- High purity thermostable recombinant FGF2-G3 protein comprising 154 aa form of FGF-2 (Uniprot: P09038) with nine stabilizing amino acid substitutions
- 17 kDa
- >98%, by SDS-PAGE quantitative densitometry
- Animal origin-free (AOF) and carrier protein-free
- Expressed in E. coli
- Manufactured in our Cambridge, UK laboratories
- Lyophilized from Tris, NaCl, CyS, mannitol
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Products are for research use only and not for diagnostic or therapeutic use.
**Vial images are representative**
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